Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos

Early embryonic development may be affected by adrenal hyperactivity in stressful situations which lead to endocrine changes the embryo environment. A sensitive period porcine is 4-cell stage when genome activation occurs. mixed vivo-in vitro system was implemented test whether an altered milieu around this could affect and blastocyst quality model. After maturation fertilisation, presumptive zygotes were exposed for 24 h plasma collected after ovulation from adrenocorticotropic hormone (ACTH)-treated, non-ACTH-treated sows; and, medium without plasma, supplemented with bovine serum albumin. Subsequently, differences gene expression tested among treatments. Cleavage rates did not differ between Blastocyst morphology assessment similar all resulting blastocysts included analysis. However, only expanded (and onwards) analysis, non-ACTH group showed better score. morphological mirrored transcription levels of various important genes whose profile significantly groups. It likely that effect environment provided ACTH-treated sows too short development. Therefore, a brief exposure have harmful consequences once fertilisation